Figure 2.

Growth assays for sensitivity of deletion mutants to increasing concentrations of NAM. Nearly 90% of mutants initially identified in the screen show greater sensitivity to 120 mM nicotinamide (NAM) compared to a wild-type control (WT). Scores for the growth in the genome-wide screen for each strain are shown in Table 1. A subset of mutants is considered highly sensitive when there is a loss of viability even at 30 mM NAM. Asterisks indicate the most sensitive mutants. Three biological replicates were done and results were similar for all three experiments. Overnight cultures of each deletion strain were serially diluted five-fold and 3 μl were spotted on indicated media and incubated at 30°C for 2–3 d.