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Growth assays reveal that conditional mutants in the essential cohesion complex subunits are sensitive to NAM. Strains with temperature sensitive mutations in the core cohesin genes (smc1-1, smc3-42, and mcd1-1) display sensitivity to nicotinamide (NAM) at permissive temperature. Overnight cultures of each strain were serially diluted fivefold and 3 μl were spotted and incubated at 30°C.
