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. 2016 Feb 12;6:21078. doi: 10.1038/srep21078

Figure 2. Testing robustness of fibril width settings.

Figure 2

A test image was created containing three bars on a black background, each with a width of 25 pixels. The bars have three different pixel intensity values: 131 to represent the TMV standard, 100 for bar A, and 200 for bar B. The entire image has gaussian noise with a standard deviation of 25 applied. Measurements were made along bars A and B in the test image using MpUL-Multi with the width measurement setting varying from 20 to 40 px and the resulting MPL measurements reported. The actual width of the test bars (25 px) is designated by a dotted line. The systematic underestimation of MPL when the specified width is too small is apparent. The measurements become more robust as the width parameter is increased beyond the actual width of the fibril. A slight underestimation of MPL at the actual width due to imperfect centering on bar B is remedied as the width grows larger than the actual fibril width.