Table 1. Assignments for peptide fragments from a trypsin digest of a 36-kDa spot*.
Experimental mass | Theoretical mass | Amino acids sequence | Position |
---|---|---|---|
1048.47 | 1048.50 | LIEYCHSK | 196–203 |
1104.61 | 1104.63 | RQDLFIVSK | 70–78 |
1105.54 | 1105.50 | LWCTFHDK | 79–86 |
1241.57 | 1241.58 | HKDYPFHAEV | 307–316 |
1503.70 | 1503.68 | HIDCAQVYQNEK | 42–53 |
1586.84 | 1586.84 | TIGVSNFNPLQIER | 156–169 |
2217.13 | 2217.09 | YKPAVNQIECHPYLTQEK | 178–195 |
*The resulting sets of peptide masses were then used to search the NCBI database for potential matches, confirming the 36-kDa spot to be aldose reductase.