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. 2016 Feb 1;27(3):442–450. doi: 10.1091/mbc.E15-10-0721

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© 2016 Heasley and McMurray This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — LEP complex mislocalization in septin mutants and septin mislocalization upon ectopic LEP expression. (A) Representative images of cells of the indicated genotypes expressing Ady3-GFP. Arrowheads, presumptive rings. (B) As in Figure 1A, for SPR3+/∆ cells carrying plasmids encoding the indicated genes. URA3, negative control. (C) Transmitted light and fluorescence micrographs (yellow fluorescent protein filter) of representative vegetative cells ectopically expressing GST-His₆-tagged Ssp1. Arrowheads, normal septin rings in cells with normal, round morphology. (D) Representative cells as in C, immunostained with anti-His₆ mAb and Alexa Fluor 594–labeled secondary antibodies.