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. 2016 Feb 1;27(3):466–482. doi: 10.1091/mbc.E15-09-0632

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© 2016 Mills et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 1: — Loss of full-length APC induces perinuclear redistribution of mitochondria. (A) APC was silenced in U2OS cells by siRNA (APC #1 and #2), and mitochondrial distribution was analyzed by immunofluorescence microscopy after cells were stained for mitochondria (CMX-Ros) and APC. The microtubule network remained intact (α-tubulin). (B) The distribution of mitochondria in different “zones” was scored (C), revealing redistribution of mitochondria to the perinuclear region (zone 1) with APC siRNAs (***, p < 0.001). (D) Loss of APC in U2OS cells was confirmed by Western blot. (E) HDF1314 cells treated with EB1 siRNA were stained for mitochondrial distribution (CMX-Ros) and EB1. Cells displaying EB1 knockdown are indicated (*). (F) Scoring of mitochondrial distribution after EB1 silencing revealed no significant difference relative to control (n.s., not significant). Bar graph data are presented as mean (±SD), statistical analysis by unpaired two-tailed t test with Bonferroni correction (C and F). Scale bars: 10 μm.