TABLE 1:

Effect of loss of APC on parameters of mitochondrial transport in migrating NIH 3T3 cells.

		Control	mAPC-red	p Value
Overall mitochondrial movement
% Motile mitochondria		48.44 ± 5.92	21.25 ± 5.32	**
% Directionality	Stationary	49.91 ± 7.47	78.76 ± 5.32	**
	Oscillating	17.08 ± 7.29	12.50 ± 4.00	n.s.
	Anterograde	24.30 ± 8.43	7.03 ± 3.39	*
	Retrograde	8.74 ± 5.43	1.70 ± 1.49	n.s.
Average displacement (μm) (normalized to 1 min)		1.19 ± 0.11	0.67 ± 0.16	**
Average distance (μm) (normalized to 1 min)		3.19 ± 0.14	2.10 ± 0.20	**
Motile mitochondria
Average velocity (μm/min) (does not include stationary time points)	Total	9.27 ± 0.39	8.82 ± 0.57	n.s.
	Anterograde	8.98 ± 0.19	8.99 ± 0.26	n.s.
	Retrograde	10.13 ± 0.76	8.75 ± 0.97	n.s.
Average distinct mitochondrial movements	Anterograde	0.23 ± 0.03	0.13 ± 0.07	**
	Retrograde	0.15 ± 0.04	0.09 ± 0.03	**
Average distance of mitochondrial movements (μm)	Anterograde	1.35 ± 0.20	1.22 ± 0.16	n.s.
	Retrograde	1.39 ± 0.18	1.16 ± 0.25	n.s.

Mitochondria in migrating 3T3 cells treated with control or mAPC-red siRNA and transfected with pGFP2-mito were tracked using MTrackJ ImageJ plug-in as in Figure 4. Based on the raw tracking data, Microsoft Excel was used to calculate movement parameters of randomly selected mitochondria, including the pool of motile mitochondria. A distinct mitochondrial movement is classified as any directional motion without pause. *, p < 0.05; **, p < 0.01; n.s., not significant. Statistical analysis by two-tailed unpaired t test.