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. 2016 Feb 1;27(3):518–534. doi: 10.1091/mbc.E15-07-0504

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© 2016 Williams et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Increased N2a cell process extension due to increased GAP-43 protein expression upon hnRNP-Q1 knockdown. N2a cells were transfected with hnRNP-Q1 #1 or Scr siRNA, GAP-43 or Scr siRNA and Lifeact-GFP and processed for immunofluorescence with GAP-43 and hnRNP-Q1 antibodies after 72 h. Cells were then imaged and categorized based on their degree of process extension (see Supplemental Figure 3A). (A) Representative images with heat maps. Scale bar: 10 μm. (B) Quantification of the results. Percent of cells in each category: Scr + Scr: cat. 1 = 84.4%; cat. 2 = 12.7%; cat. 3 = 2.6%; cat. 4 = 0.3%; Scr + GAP-43: cat. 1 = 71.6%; cat. 2 = 21.3%; cat. 3 = 6.7%; cat. 4 = 0.4%; Q1 + Scr: cat. 1 = 61.5%; cat. 2 = 21.7%; cat. 3 = 12.3%; cat. 4 = 4.5%; Q1 + GAP-43: cat. 1 = 71.2%; cat. 2 = 21.1%; cat. 3 = 6.9%; cat. 4 = 0.8%. n = 4, Scr + Scr: 379 cells; Scr + GAP-43: 239 cells; Q1 + Scr: 286 cells; and Q1 + GAP-43: 261 cells from four independent experiments. Kruskal-Wallis test, Dunn’s posthoc, p values: Scr + Scr vs. Scr + GAP-43, p = 0.0029; Scr + Scr vs. Q1 + Scr, p < 0.0001; Scr + Scr vs. Q1 + GAP-43, p = 0.0013; Scr + GAP-43 vs. Q1 + Scr, p = 0.0137; Scr + GAP-43 vs. Q1 + GAP-43, p > 0.9999; Q1 + Scr vs. Q1 + GAP-43, p = 0.0156.