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. 2016 Feb 1;27(3):535–548. doi: 10.1091/mbc.E15-06-0453

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© 2016 Patnaik, George, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 4: — Nuclear localization of villin in colon cancer cell xenografts. Colon cancer cell lines endogenously expressing villin—T-84, HT-29 16E, COLO-205, HT-29 19A, and HCT-15 cells—were grown as xenografts in SCID mice. The tumors were excised and Formalin fixed. Villin expression was determined by immunohistochemistry using a commercially available anti-villin antibody. (A) Representative images of T-84, HT-29 16E, COLO-205, and HT-29 19A. (B) Higher magnification of HCT-15 xenograft shows localization of villin in individual nuclei (arrowheads).