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. 2016 Feb 1;27(3):549–571. doi: 10.1091/mbc.E15-11-0754

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© 2016 Liu, He, et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Replicating parasites ectopically expressing mEmeraldFP-TLAP3 from a T. gondii tubulin promoter. Each panel shows a vacuole containing two intracellular parasites with nascent daughters (left) or close-to-budding daughters (right). Note that when overexpressed, TLAP3 is capable of coating the full length of the cortical microtubules, as well as of localizing to the basal complex (BC) and nascent spindle (inset, arrowhead). The inset (1×) shows a different rotational view of the region indicated by the arrow.