FIGURE 1:

APM1 and APM2 deletions have distinct sorting phenotypes. (A) APM2 but not APM1 is required for Snc1 sorting. Cell-surface levels of the Snc1 reporter GSS were quantified in yeast deletion strains by measuring invertase activity of liquid cultures normalized to wild type. Unpaired t test of mutant strains compared with wild type, ***p < 0.0001 and **p < 0.01. Error bars represent SEM (n = 6). (B) Deletion of APM1 but not APM2 bypasses the Chs3 trafficking defect of a chs6 mutant strain. Chs3 surface activity was quantified as the fluorescence intensity of strains plated on 50 μg/ml calcofluor white and reported in arbitrary units (A.U.). Unpaired t test compared with wild type, ***p < 0.0001 and *p < 0.05. Error bars represent SEM (n = 8). (C) To assess drug sensitivity, yeast were spotted in 10× dilution series and grown on YPD with Hyg B plus indicated drugs or DMSO as control.