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. 2016 Feb 1;27(3):588–598. doi: 10.1091/mbc.E15-09-0621

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© 2016 Whitfield et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 2: — Apm2 localizes to late Golgi/early endosomes. (A) Fluorescence microscopy of live endocytosis-defective sla2 yeast shows that Apm2 is not present at cell-surface clathrin-coated pits. White arrows indicate puncta that colocalize in GFP and RFP channels. (B) Kymograph analysis of Apm4-GFP or Apm2-GFP relative to cortical clathrin patches marked by Clc1-RFP. Cells were incubated with the actin inhibitor latrunculin A and imaged by TIRF microscopy. (C) Localization of Apm2-GFP relative to that of Apm1-tdTomato (AP-1 complex), Anp1-RFP (early Golgi), Sec7-DsRed (late Golgi/endosomes), and Snf7-RFP (late endosomes). Scale bar, 4 μm.