Figure 3. SMase influences the lateral mobility of LFA-1 nanoclusters.

(A) Normalized semilog distribution of D_1–4 values for LFA-1 nanoclusters in unperturbed control cells (grey) or after SMase (red) treatment. Each histogram contains at least 400 trajectories taken from >30 cells in 5 experiments. The vertical dotted lines represent the average D_1–4 values in control cells (black) and SMase treated cells (red). (B) Percentage of LFA-1 nanocluster mobility classified as immobile, slow and fast mobile in unperturbed and SMase treated monocytes. The error bars represent the mean sub-population size ± SEM of 5 independent experiments. 2way ANOVA and Bonferroni post-test were applied to the full distribution of sub-population values calculated from at least 30 cells in 3–5 experiments. P-values were compared to the respective unperturbed cells, *<0.05, **<0.01. (C,D) Square displacement plots of the slow (C) and fast (D) moving population of LFA-1 by fitting the CPD at different time lags (461–658 trajectories). Slow and fast diffusing components were fitted to a model of i) free (dashed lines) and ii) anomalous diffusion (continuous lines).