Table 1. Initial diffusion coefficients, α value and % of slow and fast sub-populations of LFA-1 nanoclusters (imaged by mAb TS2/4 Atto647N) in unperturbed, SMase treated monocytes ± addition of CytoD.

	N	α slow	D[init] slow [μm2/s]	α fast	D[init] fast [μm2/s]	% immobile	% slow	% fast
Control	658	0.67 ± 0.05	0.0045 ± 4e-05	0.86 ± 0.01	0.053 ± 0.002	21.63 ± 2.37	25.86 ± 1.62	52.51 ± 2.24
SMase	461	0.66 ± 0.04	0.0051 ± 0.003	0.74 ± 0.02	0.035 ± 0.0009	22.06 ± 2.56	31.18 ± 1.83	45.75 ± 1.96
SMase + CytoD	262	1.1 ± 0.06	0.055 ± 7e-05	0.98 ± 0.02	0.049 ± 0.001	21.51 ± 2.04	27.26 ± 2.53	50.66 ± 3.46