Figure 1.

Summary of the experimental design. We recorded data of age-specific reproductive performance and lifespan from mitonuclear introgression lines (I) and a reference population females (R) mated to the introgression line males. For the focal females, provided with a mating opportunity with four sets of reference virgin males (1–2-day-old virgin), we collected data from six age intervals. For the focal males and their reference female mates (1–2-day-old virgin), we collected data from six male age points. The symbols indicate data collected from each experimental group at each time point (open and closed symbols show which variables were recorded at a given time).