FIG 1.

The ADH2 promoter/regulatory region confers glucose-induced mRNA instability on normally stable transcripts. (A) Structures of the MAP2 and IDP1 loci after targeted integration of the ADH2 promoter. (B) ADH2, MAP2, and IDP1 transcript levels under repressed (R) and derepressed (DR) growth conditions. (C and D) Glucose-induced mRNA decay experiments were performed as described in Materials and Methods in strains W303-1a (WT), KBY127 (ADH2p-MAP2), and KBY131 (ADH2p-IDP1). (E) Levels of endogenous IDP1 and MAP2 transcripts after transcription was inhibited by the addition of 100 μg/ml 1,10-o-phenanthroline following 4 h of derepression in strain KBY77. The slopes of the linear regression curves were used to calculate the mRNA half-lives reported in Table 3.