FIG 2.
The ADH2 promoter confers SNF1-dependent mRNA expression and stability to normally SNF1-independent transcripts. (A) ADH2 and MAP2 transcript levels under repressed (R) and derepressed (DR) growth conditions. The mean results and ranges for two biological replicates are shown. (B and C) Strains KBY137 and KBY140 with the relevant genotypes [map2::natMX-ADH2p(−591 to −49)-MAP2 and idp1::natMX-ADH2p(−591 to −49)-IDP1 ura3::YIpSNF1as::URA3 snf1Δ::kanMX, respectively] were grown to mid-log phase in YP plus 5% glucose and treated as described in the legend to Fig. 1, except that 2NM-PP1 rather than glucose was added to a final concentration of 10 μM after 4 h of derepression. (D) IDP1 transcript levels in strain KBY140 after the addition of glucose or 2NM-PP1 to a 4-h derepressed culture.