FIG 3.

Primer extension analysis. (A) The 5′ ends of IDP1 mRNA were determined as described in Materials and Methods. The strains used were KBY137 (WT IDP1) and BY4741 (WT IDP1) grown under repressing (R) conditions (lanes 1 and 2, respectively) and strain KBY140 (ADH2p-IDP1) grown under derepressing (DR) conditions for 4 and 7 h (lanes 3 and 4, respectively). Lane 5, no RNA during primer extension. A 10-nucleotide (ntd) ladder is shown to the right. (B) 5′ ends of ADH2. Lane 1, RNA isolated from strain KBY140 grown under repressing conditions; lanes 2 to 4, three replicates using RNA from strain KBY140 grown for 4 h under derepressing conditions; lane 5, no RNA during the primer extension; lanes 6 and 7, two RNA preparations from strain TYY204 containing pRR01(ADR1-EV) grown under derepressing conditions for 3 h and then induced with 1 nM β-estradiol for 1 h (DR/Ind). A 10-nucleotide ladder is shown to the right.