FIG 4.

Binding sites for Adr1 are sufficient to promote glucose-induced mRNA decay. (A and B) ADH2 and lacZ transcript levels under repressed (R) and derepressed (DR) growth conditions (A), and the ratio of expression levels under derepressed and repressed growth conditions (B). (C and D) Strain W303-1a (WT) was transformed with pHDY10 (34) or pLGADH2 (35), and cultures were grown to mid-log phase in synthetic complete (SC) medium lacking uracil plus 5% glucose. (C) After 21 h of derepression, a typical glucose-induced mRNA decay experiment was performed. (D) lacZ, ACT1, and ADH2 transcript levels in strain CKY19 transformed with the lacZ expression plasmid YCpPKG2 (PYK1lacZ). After 4 h of derepression, glucose and 1,10-o-phenanthroline were added. The values for mRNA were normalized to the abundance of 18S RNA. The average results and standard deviations for four transformants of CKY19 transformed with YCpPKG2 are shown.