TABLE 2.
Chromatin immunoprecipitation analysis of RNA Pol II occupancy at the endogenous ADH2 promoter and at the ADH2p-IDP1 genea
| Culture condition | Enrichment factor (IP DNA/input) for: |
|||||
|---|---|---|---|---|---|---|
|
ADH2 |
IDP1 |
|||||
| 3′ end |
5′ end |
|||||
| Mean | Range | Mean | Range | Mean | Range | |
| DR | 12 | 7.3–16 | 12 | 9.4–15 | 11 | 8.7–13 |
| DR→R | 1.9 | 1.1–2.8 | 0.52 | 0.22–0.81 | 0.58 | 0.5–1.5 |
a
The enrichment factor is a measure of the immunoprecipitated (IP) DNA-to-input ratio at the indicated gene compared to that of a telomeric region under derepressed conditions (DR) and 30 min after adding glucose (DR→R) to 4-h cultures of strain KBY131. The means and ranges of the results from two biological samples are shown.