TABLE 3.
MAP2 and IDP1 mRNA half-lives are reduced after glucose repletion when their native promoters are replaced by the ADH2 promotera
| Genotype | mRNA | Half-life (min)b |
|---|---|---|
| WT | ADH2 | 5.1 |
| ADH2p-MAP2 | ADH2 | 4.7 |
| ADH2p-IDP1 | ADH2 | 4.1 |
| WT | MAP2 | 60 |
| ADH2p-MAP2 | MAP2 | 9.0 |
| WT | IDP1 | 60 |
| ADH2p-IDP1 | IDP1 | 14 |
a
The strain names and a description of the glucose-dependent mRNA decay experiment are in the legend to Fig. 1.
b
Half-lives for ADH2, ADH2p-IDP1, and ADH2p-MAP2 mRNAs were estimated from the data shown in Fig. 1C and D. The data for the 5-, 10-, 20-, and 30-min time points were plotted on a semilog scale, and the half-lives were calculated from the slopes of the decay curves obtained using linear regression in excel. For MAP2 and IDP1, the data shown in Fig. 1E were used in a similar manner.