FIG 5.

Monorhamnolipids reduce S. constellatus biofilm viability in mixed culture with P. aeruginosa on CF airway cells. (A) Viability of S. constellatus 7155 grown as mixed biofilms on CF airway cells with the wild type or the rhlA, rhlB, or rhlC mutant of P. aeruginosa not treated with tobramycin or treated with 5 μg/ml tobramycin. (B) Surfactant score from assays of mixed biofilms of S. constellatus 7155 with the wild type or the rhlA, rhlB, or rhlC mutant of P. aeruginosa on CF airway cells not treated with tobramycin or treated with 5 μg/ml tobramycin. The dashed line indicates the limit of detection. (C) Viability of the wild type or the rhlA, rhlB, or rhlC mutant of P. aeruginosa grown as mixed biofilms on CF airway cells with S. constellatus 7155 not treated with tobramycin or treated with 5 μg/ml tobramycin. For all panels, the bars and error bars indicate the means and SD of the results from three biological replicates. *, P < 0.05; **, P < 0.01, as determined by an unpaired t test.