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Production of Bsp22 and pertactin (Prn) in B. pertussis strains UT25Sm1 and PMK21. B. pertussis strains were cultured in SSC (lanes 1 and 5), SSC-0.5E (lanes 2 and 6), SSC-0.2E (lanes 3 and 7), and SSC-0E (lanes 4 and 8) medium. At 30 h after starting the culture, protein samples were prepared and separated by 12.5% polyacrylamide SDS-PAGE gels. Prn and Bsp22 were detected by immunoblotting as described in Materials and Methods.
