FIG 1.

Temporal regulation of the σ²⁸- and σ⁶⁶-dependent promoters of dnaK and bioY. 5′ RACE analysis of transcription from the σ²⁸-dependent P2 promoter (A) and σ⁶⁶-dependent P1 promoter (B) of dnaK and bioY performed on RNA extracted from C. trachomatis-infected cells at 14 and 24 hpi. Promoter-specific PCR products were resolved on a 2% agarose gel. Each band was excised and sequenced to confirm that the PCR product originated from the chlamydial promoter.