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SouR binding to promoters of potential regulon members. Shown are EMSAs with purified MBP-SouR and biotinylated probes of promoter regions from operons induced by sarcosine. Each blot represents a separate biotinylated probe, with dhcA included as a negative control. The MBP-SouR concentrations are shown below the lanes. The data are representative of the results of at least three independent experiments with two separate batches of purified MBP-SouR.
