FIG 6.

Roles of SouR- and sarcosine-regulated genes during growth on sarcosine. Shown are the culture densities (OD₆₀₀) after 24 h of growth in MOPS minimal medium without nitrogen supplemented with either 20 mM pyruvate and 10 mM ammonium chloride (top) or 40 mM sarcosine (bottom) for the transposon insertion mutants labeled on the x axis. The dhcA insertion mutant is known not to have a role in this pathway and served as the positive growth control (no growth defect). Statistical significance was determined using one-way ANOVA with Dunnett's posttest, with growth in the dhcA mutant as the comparator for all other data. n.s., not significant; **, P < 0.01; ***, P < 0.001. The data shown are summaries of the results of three independent experiments, each with three biological replicates; the error bars represent standard errors of the means.