Role of gfnR during growth in the presence of formaldehyde (form). Shown are the culture densities (OD600) after 24 h of growth in MOPS minimal medium, 20 mM sodium pyruvate, and 5 mM d-glucose in the presence (+) and absence (−) of 0.75 mM formaldehyde for wild-type, ΔgfnR, ΔgfnR attTn7::gfnR, and ΔgfnR attTn7::EV (empty-vector) strains. The data are representative of the results of three separate experiments, and the error bars represent standard deviations.