TABLE 2.
Effects of souR and gfnR mutations on sarcosine regulation of regulon members
| Transcript | Relative expressiona in: |
||
|---|---|---|---|
| ΔgbdR strain | ΔgbdR ΔsouR strain | ΔgbdR ΔgfnR strain | |
| soxA | 32.9 (8.0)b | 1.11 (0.2) | 31.0 (11.0)b |
| adhC | 3.60 (2.8)d | 1.23 (0.2) | 1.21 (0.3) |
| sdaB | 5.40 (2.4)c | 0.73 (0.1) | Not determined |
a
Relative expression was calculated based on the expression in the WT in the pyruvate control normalized to the rplU transcript; standard deviations are in parentheses. The data were analyzed using one-way ANOVA within each transcript using a Dunnett's corrected posttest with the pyruvate condition as the comparator; unmarked relative expression numbers are not statistically significant.
b
P < 0.01.
c
P < 0.05.
d
The adhC transcript is stochastically induced under these conditions, and while not different using the above-mentioned parametric analysis, the data are not normally distributed. Analysis with the nonparametric Mann-Whitney test showed significance (P = 0.026).