TABLE 4.
Self-immunity of the recombinant strains expressing different gene combinations to enterocin NKR-5-3B
| Strain or relevant plasmida | MIC (μM)b | Immunityc |
|---|---|---|
| E. faecalis JH2-2 | 0.826 | − |
| pMG36c (vector control) | 0.826 | − |
| pNK-B | 0.826 | − |
| pNK-B4 | >423 | ++ |
| pNK-B3 | 0.826 | − |
| pNK-B2 | 1.652 | − |
| pNK-B1 | 0.826 | − |
| pNK-B34 | >423 | ++ |
| pNK-B24 | >423 | ++ |
| pNK-B23 | 0.826 | − |
| pNK-B14 | >423 | ++ |
| pNK-B13 | 211.5 | + |
| pNK-B12 | 0.826 | − |
| pNK-B234 | >423 | ++ |
| pNK-B134 | >423 | ++ |
| pNK-B124 | >423 | ++ |
| pNK-B123 | 211.5 | + |
| pNK-B1234 | >423 | ++ |
a
E. faecalis JH2-2 was used as a host strain. Strains carrying the indicated plasmids were assayed as described in Materials and Methods.
b
MIC for the indicated strain or the strain expressing the indicated plasmid. The highest concentration of Ent53B used was 423 μM.
c
++, +, and − represent strong immunity, moderate immunity, and no immunity, respectively.