TABLE 3.
Oligonucleotides used in this study
| Oligonucleotide | Use | Sequence (5′ → 3′) |
|---|---|---|
| P148 | Cloning of lexA region | CGAGCAAGGCATGTTCACAG |
| P149 | Cloning of lexA region | CGATCTCATCCAGAATGTCG |
| P150 | Construction of ΔlexA::aph1 allele | GCGATGCATCGGCTTCATGAGTCACCTGTC |
| P151 | Construction of ΔlexA::aph1 allele | GCGATGCATCGCAACAGCACTTGGATGTG |
| P309 | Construction of lexA (Ind−) allele | GTGAACCGATTCTTGCTCAAG |
| P310 | Construction of lexA (Ind−) allele | CATCGGCAACGCGGCCAATCAAAGGC |
| P359 | Cloning of ssb | CGGGAATTCACCATGGCAAGCCGTGGCGTGAAC |
| P360 | Cloning of ssb | CCGCTGCAGCTAAAACGGGATGTCGTCATC |
| P367 | PrecN-lacZec fusion | CGCGTCGACGCTTGGCTGGTCGAGCAAAC |
| P368 | PrecN-lacZec fusion | CGCCTGCAGCATGTTTGCACCTGTATGAT |
| P152 | Deletion of recA | TGTGACCCACGATCAGGATG |
| P153 | Deletion of recA | CAGTTGCAGAACGTCAGTC |
| P172 | Deletion of recA | CCGTCTAGACGTTAATCAGTTTGCGGGTGAGTGGC |
| P173 | Deletion of recA | GGCGAGCTCCCGACGTTTTCTAGGTCGTTG |