TABLE 4.
Integration frequencies of CTXϕ and its RS2 elements in wt, ΔlexA, and lexA (Ind−) V. cholerae reporter strainsa
| IMEXb | Host | % integration (mean ± SD)c | No. of screened colonies |
|---|---|---|---|
| CTXϕ | BS1 | 5.46 ± 0.44 | 393 |
| RS2 | BS1 | 11.6 ± 1.36 | 697 |
| CTXϕ | BS20 (ΔlexA) | 2.48 ± 1.5 | 850 |
| RS2 | BS20 (ΔlexA) | 3.86 ± 1.5 | 381 |
| CTXϕ | BD14a [lexA (Ind−)] | 6.34 ± 1.4 | 609 |
| RS2 | BD14a [lexA (Ind−)] | 13.97 ± 1.39 | 345 |
a
V. cholerae cells carrying replicative or integrative forms of CTXϕ or RS2 form blue and white colonies on the selection plate. The selection plate contained the chromogenic substance X-Gal and Str and Cam antibiotics.
b
IMEX, integrative mobile element exploiting Xer recombination.
c
The integration frequency was estimated by counting blue and white colonies. The standard deviation was calculated from at least three independent experiments.