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Identification of NirK, cytochrome c5, and CcoP as N. elongata subsp. glycolytica glycoproteins. C-terminally 6-His-tagged proteins were affinity purified from wild-type (wt) and pglC backgrounds and subjected to immunoblotting with a tetra-His epitope-recognizing antibody. Strains used were nirK-His (KS992), nirK-His pglC (KS994), cycB-His (KS997), cycB-His pglC (KS1005), ccoP-His (KS996), and ccoP-His pglC (KS1003) strains.
