FIG 2.
Complementation of the trkH and trkA mutants. (A) Expression of a PrsmB::gfp promoter fusion was measured in WT bacteria harboring the control vector (pOM18), the trkH::Tn5 mutant carrying either the pOM18 empty vector or the vector expressing trkH, p(trkH+), and the trkA::Tn5 mutant carrying either the pOM18 empty vector or the vector expressing trkA, p(trkA+). Fluorescence was measured in cells collected from cultures grown to an OD600 of 0.4 in MM. (B) Pel activity was measured in cell-free supernatants from cultures of the bacterial strains indicated above grown in LB supplemented with PGA at an OD600 of 0.4. Error bars represent standard errors of the means (SEM); n = 6.