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Mutants in the Trk system are impaired in virulence. The virulence of WT bacteria and trkH::Tn5 and trkA::Tn5 mutants was measured by quantification of the mass of potato tuber maceration induced by these bacteria 48 h after inoculation of the potato tubers. Potatoes were inoculated with approximately 3 × 105 cells of the respective strain grown overnight in LB. **, P < 0.01; n = 7. This is a representative experiment from three independent experiments.
