FIG 1.

Analyses of ppGpp⁰ Δlon mutant synthetic lethality and its suppression. (A) Retention or loss of pRCspoT plasmid was assessed by blue and white colony color in LB agar plates containing X-Gal and IPTG at 37°C. All strains carried pRCspoT, and their relevant genotypes are indicated above each image. The fraction of white colonies obtained and the actual number of white colonies and total colonies used for calculation are shown in parentheses below each image. (B) Growth of ppGpp⁰ Δlon/pRCspoT strain (AN519) in LB Amp medium with or without IPTG. Arrows indicate time points at which aliquots were taken for microscopic analysis of cells (shown in Fig. 3 and Table 1). Details about growth conditions are given in the microscopy section in Materials and Methods.