TABLE 2.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli NEB 5-alpha | DH5α derivative | New England Biolabs |
| Cyanobacteria | ||
| Synechocystis sp. PCC 6803 | Wild type | Lab collection |
| Δ97 mutant | Wild type transformed with pBE97; sll0158Δ97 Kmr | This study |
| Δ112 mutant | Wild type transformed with pBE112; sll0158Δ112 | This study |
| Δ97x mutant | Wild type transformed with pBE97 and pGlgX2-8801; sll0158Δ97 Kmr slr0237::PCC8801_1045 Kmr Spr | This study |
| Δ112x mutant | Wild type transformed with pBE12 and pGlgX2-8801; sll0158Δ112 slr0237::PCC8801_1045 Kmr Spr | This study |
| Cyanothece sp. ATCC 51142 | Wild type | Lab collection |
| Plasmids | ||
| pRL448 | Plasmid carrying Kmr cassette | 42 |
| pRL453 | Plasmid carrying Sp/Sm Ω cassette | 42 |
| pBE97 | 97-amino-acid truncation construct for PCC 6803 BE containing a Kmr cassette | This study |
| pBE112 | 112-amino-acid truncation construct for PCC 6803 BE containing a Kmr cassette | This study |
| pGlgX2-8801 | Construct with glgX2 from PCC 8801 flanked by slr0237 fragments and for Sp/Sm Ω cassette recombination | This study |