TABLE 5.
Effects of glycogen branching- and debranching-enzyme modifications on glycogen storage and structurea
| Strain | DPn | DPw | % increase of glycogen content after 72 h in LNc BG-11 |
|---|---|---|---|
| Synechocystis sp. PCC 6803 | 8.9 ± 0.1 | 11.5 ± 0.3 | NA |
| Δ97 mutant | 9.6 ± 0.1 | 12.6 ± 0.1 | ∼27 |
| Δ112 mutantb | 9.3 | 12.4 | ∼22 |
| Δ112x mutantb | 9.4 | 12.8 | ∼4 |
| Cyanothece sp. ATCC 51142b | 11.3 | 14.5 | NA |
a
Values are means ± standard deviations obtained from three independent experiments. Average chain length was calculated based on peak area of each chain on HPAEC chromatograms. NA, not available (not measured).
b
Only one experiment was performed.
c
LN, low nitrate (0.01× nitrate).