FIG 2.

Regulatory elements encoded within the yscW-lcrF sequence. Nucleotide sequences of the yscW-lcrF promoter regions for Y. pestis CO92, Y. enterocolitica 8081, and Y. pseudotuberculosis YPIII were aligned using ClustalW2. Nucleotides whose sequences are not identical are marked in red, while the conserved nucleotides are indicated by asterisks. The identified binding sequence for IscR is marked in purple, and critical residues required for IscR binding within this motif are in bold (28, 64). The binding site for RcsB is marked in green (71). The region experimentally determined to be involved in YmoA/H-NS binding, marked in brown, is downstream of the transcriptional start site (49). The sequence encoding the RNA thermometer is marked in yellow and is encoded within the intergenic region between yscW and lcrF (49). The DNA bending region identified within the yscW-lcrF operon is denoted with a dotted line and is within the intergenic region between yscW and lcrF (59). The −10 and −35 boxes, the transcriptional start site, the ribosome binding sites (rbs) upstream of the yscW and lcrF coding sequence, and the fourU element in the intergenic region between the yscW and lcrF coding regions are marked in bold (49). All coding sequences are marked in blue.