FIG 2.
Transposon library mutants (VFS strains), but not their backcrossed-Tn counterparts (TME strains), have phenotypes consistent with deficient ainS pheromone signaling. (A) PainS-gfp transcriptional reporter activity in cultures grown in SWTO medium to an OD595 of ∼2.5. (B) Peak specific luminescence of cultures grown in SWTO medium. (C) Strain motility as measured by increase in spot diameter (on 0.25% agar plates) over 24 h. (D) C8-AHL extracted and bioassayed from cultures grown in SWTO medium. ND, none detected. In each panel, data are from single representative experiments of three independent experiments. Error bars on all panels indicate standard errors (n = 3). *, P < 0.01, and **, P < 0.001, compared to ES114.