FIG 7.

Competitive advantage of luxO* mutants relative to ES114 and litR mutants during prolonged coculture. ES114 (A to D) or litR mutant JB18 (E to H) were mixed with JHK057 [luxO(V106A)] in ratios of 1:1, 10:1, or 100:1, with JHK057 always at a disadvantage. Mixes were diluted in 24-well microtiter plates and grown statically for 96 h. At intervals of 0, 24, 48, and 96 h, wells were thoroughly mixed and dilution plated to determine the ratios of viable CFU for each strain in the mixture based on translucence (JHK057 versus ES114) or Erm resistance (JHK057 versus JB18). Data are from a single representative experiment of three independent experiments. Error bars indicate standard errors (n = 3).