TABLE 2.
Summary of luxO* mutants described in this study
| Strain | luxO* mutation | LuxO* amino acid substitution | luxO* crossed into ES114 | luxO* function assessed in transa |
|---|---|---|---|---|
| VFS002F6-T | C272A | A91D | X | X |
| VFS012E9-T | C122T | P41L | X | X |
| VFS014F5-T | T317C | V106A | X | X |
| VFS014B6-T | T317G | V106G | X | X |
| VFS021D9-T | C1004T | T335I | X | X |
| WTTG0 | C320G | T107R | ||
| WTTG1 | T281G | F94C | ||
| WTTG2 | G341C | R114P | ||
| WTTG3 | A971G | H324R | ||
| WTTG4 | C293A | P98Q | ||
| WTTG5 | G338T | R113L | ||
| WTTG12 | C293T | P98L | ||
| WTTG17 | T323G | V108G | ||
| WTTG20 | T317G | V106G | ||
| WTTG21 | T317G | V106G | ||
| WTTG22 | A956G | H319R | ||
| WTTG24 | C613T | L205F |
a
luxO genes were cloned into the low-copy-number vector pVSV105, and their ability to affect ainS-controlled phenotypes (luminescence as well as PainS and Pqrr reporter activity) in the ΔluxO strain TIM306 was confirmed (24).