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The ActiveX serine protease probe binds to LipA. Culture supernatants isolated from overnight WT/plipBA and ΔgspD/plipBA cultures induced with increasing concentrations (μM) of IPTG were incubated with the ActiveX serine protease probe for 1 h. Samples were subjected to SDS-PAGE and Typhoon image analysis as described in Materials and Methods. A molecular mass marker is shown on the right.
