TABLE 1.
Strains used in this studya
| Strain or plasmid | Genotype or description | Source, construction, or reference |
|---|---|---|
| Strain | ||
| Escherichia coli | ||
| DH5α | F− Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 supE44 thi-1 gyrA96 relA2 | Life Technologies |
| Pectobacterium carotovorum subsp. carotovorum | ||
| SR38 | Aggressive soft-rot pathogen isolated from a shipment of Florida tomatoes | Laboratory collection, from J. Bartz |
| Salmonella enterica serovar Typhimurium | ||
| 14028 | Wild type | American Type Culture Collection |
| JS246 | 14028 yjeP::res1-tetRA-res1 | 26 |
| JTN215 | 14028 kdgR27::kan | Made by Datsenko-Wanner (26) mutagenesis using primers JTN177 and JTN178; verified with primers JTN179 and JTN180 |
| AG32 | 14028 kdgR27::frt res1-tetRA-res1 | AG1 × P22/JS246 |
| AG1 | 14028 kdgR27::frt | Made by electroporating pCP20 into JTN215 |
| AG2 | 14028 kdgR27::tnpR-lacZY | Made by electroporating pCE70 into AG1 |
| AG3 | 14028 kdgR2::tnpR-lacZY res1-tetRA-res1 | AG2 × P22/JS246 |
| AG15 | 14028 kdgK15::frt-kan-frt | Made by Datsenko-Wanner (26) mutagenesis using primers JTN163 and JTN164; verified with primers JTN165 and JTN166 |
| AG19 | 14028 kdgK15::frt | Made by electroporating pCP20 into AG15 |
| AG27 | 14028 kdgK15-tnpR-lacZY | Made by electroporating pCE70 into AG19 |
| AG28 | 14028 kdgK15-tnpR-lacZY res1-tetRA-res1 | AG27 × P22/JS246 |
| AG38 | 14028 kdgR27::frt kdgK15::tnpR-lacZY res1-tetRA-res1 | AG32 × P22/AG27 |
| AG25 | 14028 kduID45::frt-kan-frt | Made by Datsenko-Wanner (26) mutagenesis using primers JTN167 and JTN168; verified with primers JTN169 and JTN170 |
| AG31 | 14028 kduID45::frt | Made by electroporating pCP20 into AG25 |
| AG33 | 14028 kduID45::tnpR-lacZY | Made by electroporating pCE70 into AG31 |
| AG36 | 14028 kduID45::tnpR-lacZY res1-tetRA-res1 | AG33 × P22/JS246 |
| AG39 | 14028 kdgR27::frt kduID45::tnpR-lacZY res1-tetRA-res1 | AG32 × P22/AG33 |
| AG26 | 14028 kdgM32::frt-kan-frt | Made by Datsenko-Wanner (26) mutagenesis using primers AG7 and AG8; verified with primers AG33 and AG34 |
| AG30 | 14028 kdgM32::frt | Made by electroporating pCP20 into AG26 |
| AG34 | 14028 kdgM32::tnpR-lacZY | Made by electroporating pCE70 into AG31 |
| AG35 | 14028 kdgM32::tnpR-lacZY res1-tetRA-res1 | AG34 × P22/JS246 |
| AG41 | 14028 kdgR27::frt kdgM::tnpR-lacZY res1-tetRA-res1 | Ag32 × P22/AG34 |
| KI1 | 14028 eda42::frt-kan-frt | Made by Datsenko-Wanner (26) mutagenesis using primers AG92 and AG93; verified with primers AG94 and AG95 |
| AG42 | 14028 eda42::frt | Made by electroporating pCP20 into KI1 |
| AG45 | 14028 eda42::tnpR-lacZY | Made by electroporating pCE71 into AG42 |
| AG46 | 14028 eda42::tnpR-lacZY res1-tetRA-res1 | AG45 × P22/JS246 |
| AG47 | 14028 kdgR27::frt eda42::tnpR-lacZY res1-tetRA-res1 | AG32 × P22/AG45 |
| AG44 | 14028 kdgT44::frt-kan-frt | Made by Datsenko-Wanner (26) mutagenesis using primers AG64 and AG65; verified with primers AG66 and AG67 |
| AG47 | 14028 kdgK15::frt kduID::frt-kan-frt | AG19 × P22/AG25 |
| AG48 | 14028 kdgK15::frt kduID::frt | Made by electroporating pCP20 into AG47 |
| AG49 | 14028 kdgK15::frt kduID45::frt kdgM32::frt-kan-frt | AG48 × P22/AG26 |
| Plasmid | ||
| pKD4 | frt-kan-frt template | 26 |
| pCP20 | FLP+ l cI857+ l PR Repts Ampr Cmr | 26 |
| pCE70 | FRT-tnpR-lacZY oriR6K (kan); contains wild-type tnpR promoter, FRT orientation A | 27 |
| pCE71 | FRT-tnpR-lacZY oriR6K (kan); contains wild-type tnpR promoter, FRT orientation B | 27 |
| pKD46 | λ Red+ (Ampr pSC101 oriTS) | 26 |
a
FRT, FLP recombination target.