FIG 2.

2HP-inducible proteins. (A) Rhodococcus rhodochrous PY11 was cultivated in minimal medium supplemented with 0.1% succinate (lane S) or 0.1% 2HP as a single source of carbon (lane 2HP). The positions of molecular mass markers are shown (in kilodaltons) to the left of the gels. (B) Purification of 2HP-inducible proteins (lane 1) and determination of N-terminal sequences. SDS-polyacrylamide gels were stained with Coomassie blue. Arrows, 2HP-inducible proteins. The positions of molecular mass markers (lane 2) are shown (in kilodaltons) to the right of the gels. (C) Genetic organization of the hpo gene locus, which is involved in the metabolism of 2HP in Rhodococcus rhodochrous PY11, and its flanking regions. Black arrows, genes that are known to be involved in 2HP degradation; gray arrows, transport system and regulatory gene; white arrows, other open reading frames (ORFs) in the locus.