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Immunoblots showing Cry5B and truncated Cry5B (tCry5B) expression, constitutively in log-phase cultures (vector, pTRK593; Cry5B, pTRK1040; and tCry5B, pTRK1041) of E. coli and L. lactis MG1363 and KP1 and leakily from a leaky vector (pTRK1061) in L. lactis KP1 (Cry5B, pTRK1062; and tCry5B, pTRK1063). (A) Cell lysates. (B) Cell-free supernatants.
