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Nisin induction of Cry5B expression from Lactococcus lactis. Controls were L. lactis MG1363 and KP1 with vector (pTRK593) and with constitutive expression of Cry5B (pTRK1040). Nisin-induced expression of Cry5B was from MG1363 with pTRK1068, with increasing concentrations of nisin as indicated by triangles. Samples were loaded at different protein concentrations (2.5, 5, or 10 μg protein per lane).
