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SDS-PAGE analysis of the apparent molecular masses of wild-type and mutant phytases produced in P. pastoris. Lanes: 1 and 12, protein markers (M); 2, 11 and 15, the nonglycosylated wild-type (WT) YeAPPA and mutants YkAPPA-S200A and YrAPPANLT397-399DMK; 3 to 10, 12 and 13, the N-glycosylated YeAPPA-A200S, YeAPPA-DMK397-399NLT, YkAPPA, YkAPPA-D397N/K399T, and YrAPPA before (−) and after (+) N-deglycosylation with endo H, respectively.
