FIG 1.
Experimental design of in vitro selection assays with enrofloxacin, using PG45T as an example. The selected clones are named by a letter followed by incremental numbers corresponding to successive experimental steps. The diamond symbols represent clones with no mutation, while clones with alterations in ParC, GyrA, and both loci are shown as triangles, squares, and circles, respectively. The numbers at nodes and arrow extremities indicate the number of clones isolated for each step and their distribution within the different genotypes. The enrofloxacin concentration for each step is indicated on the left. MIC assays were performed for all PG45T-derived clones except A2, A3, and A4. Dotted arrows indicate experimental steps with no further selection passages.