. 2016 Feb 5;82(4):1060–1068. doi: 10.1128/AEM.03280-15

TABLE 1.

Relationships between MIC values and mutations in gyrA, gyrB, parC, and parE QRDRs of M. bovis clinical isolates

Isolate name or characteristic	Origin, no. of isolates, yr of isolation	MIC (value or range) (μg/ml)^a			QRDR-encoded substitution^b
		MIC (value or range) (μg/ml)^a			No. of isolates with phenotype/total no. of isolates	GyrA	GyrB	ParC
		ENR	DAN	MAR	No. of isolates with phenotype/total no. of isolates	Ser83	Asp362	Ser80	Asp84
PG45^T (control)	USA, 1, 1962	0.25	0.25	0.5	1/1
Old	France, 11, 1978–1983	0.125–0.25	0.125–0.25	0.25–1	11/11
Recent	France, 20, 2009–2012	0.25–0.5	0.5–1	0.5–2	17/20		Asn
		0.5	1	2	1/20		Asn	Ile^d
		0.5–1	0.5–1	2^c	2/20		Asn		Tyr^d
Contemporary^e	France, 21, 2013–2014	0.25–0.5	0.5–1	0.5–2	20/21		Asn
		16	16	>64	1/21	Phe^d	Asn	Ile^d
Control	Switzerland, 2, 2010–2011	0.125^c	0.25^c	0.5^c	2/2		Asn

ENR, enrofloxacin; DAN, danofloxacin; MAR, marbofloxacin.

The genotype of PG45^T was used as a reference, and E. coli amino acid numbering was used. There were no mutations in ParE.

Same MIC values for both strains.

Mutation already described for M. bovis isolates in other countries (10, 12).

Data in bold refer to the recently detected clinical isolate with high-level resistance to fluoroquinolones.