TABLE 1.
Target | Forward primer (5′–3′) | Reverse primer (5′–3′) | Size (bp)a | Purpose |
---|---|---|---|---|
3′-ALP | CTGTACGAGCAGAGCCAG | Oligo(dT) | 3′ RACE | |
3′-APN1 | TGGTCTTCGTGGTGG | Oligo(dT) | 3′ RACE | |
3′-APN2 | TGGTCTTCGTAATGG | Oligo(dT) | 3′ RACE | |
3′-APN3 | TGGTCTTCGCCAAGG | Oligo(dT) | 3′ RACE | |
3′-APN4 | TGGTCTTCGTCATGG | Oligo(dT) | 3′ RACE | |
3′-APN5 | TGGTCTTCGTGAGGG | Oligo(dT) | 3′ RACE | |
3′-ABCC2 | TATCAATAATTCCGCAAGAGCCAGTG | Oligo(dT) | 3′ RACE | |
SfL18 | CGTATCAACCGACCTCCACT | AGGCACCTTGTAGAGCCTCA | 126 | qRT-PCR |
Sfcadherin | GAGAGCTGAGGGTCACTTGG | AGCGGACTCGGTTGTAGAGA | 299 | qRT-PCR |
SfmALP1 | CACTGCCGCTACTGTGCTG | CCTGTGCCTTATCATTCCAAA | 125 | qRT-PCR |
SfmALP2 | GGCTTTCTGCCCAACTGT | TCTACGAGCCAATCAACG | 114 | qRT-PCR |
SfsALP1 | ACGAGCGAGACGTGTATCACAA | CGCCCAGGAACATGACCAC | 177 | qRT-PCR |
SfAPN1 | TCTCAGTTTCTTCACTTTGCTA | ACTTGGGCAAAGGTGTTC | 127 | qRT-PCR |
SfAPN3 | TCTCAGGCAATGAAGCCAATA | CACCCATGCTTTGAAATCCTC | 167 | qRT-PCR |
SfAPN4 | GAAGTGGTTCCCCTGCTA | CGAGACGACAACGACATG | 238 | qRT-PCR |
SfAPN6 | ATCTTGGGACCGATTCTA | TTGTCATGGGACCTAACT | 255 | qRT-PCR |
SfABCC2 | CCTCAGACGGATGCTTTG | GTCGCCTGTTTCCTTCAC | 210 | qRT-PCR |
SfmALP1 | TTGTGGAAGGCGGTCGCATCGAC | Oligo(dT) | 3′ RACE | |
SfmALP1 | Kit-specific adaptor primer | GCATGCCATCCCACGTGTCATTCG | 5′ RACE | |
SfmALP1 | GGTACCATGGGGTCGTTGAGCTTGCTAT | AGATCTTCAGTGATGGTGGTGATGGTGTTTCACACTAAAGGAAGCCAAC | 1,635 | Full-length cloning |
SfmALP2 | GTGGAGAACGATGGCTTCGACACGG | Oligo(dT) | 3′ RACE | |
SfmALP2 | Kit-specific adaptor primer | GCGCTAGCTCTACCCAGTTGCGATGGT | 5′ RACE | |
SfmALP2 | GGATCCACCATGAGGTCGCTACTGACTTA | CTCGAGTCAGTGGTGATGGTGATGATGTTGTTGGAATAGTGAAG | 1,626 | Full-length cloning |
SfsALP1 | CACGCCTACCCCGGGGACCACTTCAA | Oligo(dT) | 3′ RACE | |
SfsALP1 | Kit-specific adaptor primer | GGACATGACGTGCGCGTGGTCCG | 5′ RACE | |
SfsALP1 | GGTACCATGTCTGCCCGCGCCCACGC | CTCGAGTCAGTGGTGATGGTGATGATGGTGTCGGCAGGCGTGTC | 1,572 | Full-length cloning |
The expected PCR product size is stated when available.