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. 2016 Feb 5;82(4):1023–1034. doi: 10.1128/AEM.02871-15

TABLE 1.

List of primers used in this study

Target Forward primer (5′–3′) Reverse primer (5′–3′) Size (bp)a Purpose
3′-ALP CTGTACGAGCAGAGCCAG Oligo(dT) 3′ RACE
3′-APN1 TGGTCTTCGTGGTGG Oligo(dT) 3′ RACE
3′-APN2 TGGTCTTCGTAATGG Oligo(dT) 3′ RACE
3′-APN3 TGGTCTTCGCCAAGG Oligo(dT) 3′ RACE
3′-APN4 TGGTCTTCGTCATGG Oligo(dT) 3′ RACE
3′-APN5 TGGTCTTCGTGAGGG Oligo(dT) 3′ RACE
3′-ABCC2 TATCAATAATTCCGCAAGAGCCAGTG Oligo(dT) 3′ RACE
SfL18 CGTATCAACCGACCTCCACT AGGCACCTTGTAGAGCCTCA 126 qRT-PCR
Sfcadherin GAGAGCTGAGGGTCACTTGG AGCGGACTCGGTTGTAGAGA 299 qRT-PCR
SfmALP1 CACTGCCGCTACTGTGCTG CCTGTGCCTTATCATTCCAAA 125 qRT-PCR
SfmALP2 GGCTTTCTGCCCAACTGT TCTACGAGCCAATCAACG 114 qRT-PCR
SfsALP1 ACGAGCGAGACGTGTATCACAA CGCCCAGGAACATGACCAC 177 qRT-PCR
SfAPN1 TCTCAGTTTCTTCACTTTGCTA ACTTGGGCAAAGGTGTTC 127 qRT-PCR
SfAPN3 TCTCAGGCAATGAAGCCAATA CACCCATGCTTTGAAATCCTC 167 qRT-PCR
SfAPN4 GAAGTGGTTCCCCTGCTA CGAGACGACAACGACATG 238 qRT-PCR
SfAPN6 ATCTTGGGACCGATTCTA TTGTCATGGGACCTAACT 255 qRT-PCR
SfABCC2 CCTCAGACGGATGCTTTG GTCGCCTGTTTCCTTCAC 210 qRT-PCR
SfmALP1 TTGTGGAAGGCGGTCGCATCGAC Oligo(dT) 3′ RACE
SfmALP1 Kit-specific adaptor primer GCATGCCATCCCACGTGTCATTCG 5′ RACE
SfmALP1 GGTACCATGGGGTCGTTGAGCTTGCTAT AGATCTTCAGTGATGGTGGTGATGGTGTTTCACACTAAAGGAAGCCAAC 1,635 Full-length cloning
SfmALP2 GTGGAGAACGATGGCTTCGACACGG Oligo(dT) 3′ RACE
SfmALP2 Kit-specific adaptor primer GCGCTAGCTCTACCCAGTTGCGATGGT 5′ RACE
SfmALP2 GGATCCACCATGAGGTCGCTACTGACTTA CTCGAGTCAGTGGTGATGGTGATGATGTTGTTGGAATAGTGAAG 1,626 Full-length cloning
SfsALP1 CACGCCTACCCCGGGGACCACTTCAA Oligo(dT) 3′ RACE
SfsALP1 Kit-specific adaptor primer GGACATGACGTGCGCGTGGTCCG 5′ RACE
SfsALP1 GGTACCATGTCTGCCCGCGCCCACGC CTCGAGTCAGTGGTGATGGTGATGATGGTGTCGGCAGGCGTGTC 1,572 Full-length cloning
a

The expected PCR product size is stated when available.